Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1935065 | Biochemical and Biophysical Research Communications | 2009 | 4 Pages |
Abstract
A major feedback mechanism of cholesterol in transcription of cholesterol metabolism-related genes is mediated by sterol regulatory element-binding protein (SREBP). Involvement of glycosphingolipids (GSLs) in the SREBP pathway is unknown. In this study, we examined the effects of GSL depletion on SRE-mediated gene transcription using GSL-defective cells. We found that the content of mature SREBP, the transcriptional active form, is increased in the GSL-defective cells. Transcription of SREBP target genes and cholesterol synthesis are also induced in the GSL-defective cells. These results indicate that GSL deficiency up-regulates the SREBP pathway, pointing out the regulatory role of GSL in cholesterol homeostasis.
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Authors
Reiko Ishitsuka, Yoshio Hirabayashi, Toshihide Kobayashi,