Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1935074 | Biochemical and Biophysical Research Communications | 2009 | 5 Pages |
Abstract
ErbB3 transactivation can make tumor cells resistance to ErbB1/ErbB2 targeting drugs. This urges for a reliable method to determine cell surface ErbB3 levels, but in our hands iodinated NRG1β is unstable and tends to underestimate the number of ErbB3 receptors in a radio-receptor assay. Here we show by the use of a radio-labeled high affinity neuregulin mutant NRG/YYDLL that ErbB3 levels can be determined in a reliable manner by Scatchard analysis. Furthermore we show by differential competition with unlabeled NRG/YYDLL and betacellulin that the number of ErbB3 and ErbB4 receptors can be quantified separately on cultured human breast cancer cells.
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Authors
Sebastian P. van der Woning, Everardus J.J. van Zoelen,