Article ID Journal Published Year Pages File Type
1935412 Biochemical and Biophysical Research Communications 2008 6 Pages PDF
Abstract

The phosphorylated derivative of the immunosuppressant FTY720 interacts with and modulates the function of sphingosine-1-phosphate (S1P)-receptors. We observed a significant reduction of endothelial surface binding of a S1P1-specific antibody after FTY720 treatment of 6 h and longer, which was associated with a reduced healing after mechanic injury, impaired angiogenesis and enhanced adhesion molecule expression. FTY720 (5 h) had no impact on the expression of S1P1- or S1P3-encoding transcripts. Notably, pre-treatment of cells with FTY720 for only 30 min, which did not reduce S1P1 surface expression, inhibited the rapid S1P- and SEW2871- (a S1P1 agonist) induced cortical actin formation and cell migration. FTY720 was effective at concentrations as low as 5 nM.FTY720 at therapeutic concentrations may be harmful by impairing important endothelial functions. Interestingly, FTY720 inhibited endothelial actin remodelling and cell migration without decreasing S1P1 surface expression.

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