Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1935565 | Biochemical and Biophysical Research Communications | 2008 | 6 Pages |
Abstract
An unnatural base pair between 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) and pyrrole-2-carbaldehyde (Pa) could expand the genetic alphabet and allow the incorporation of non-standard amino acids into proteins at defined positions. For this purpose, we synthesized tRNAs bearing Pa at the anticodon and tested non-standard amino acid phosphoserine aminoacylation by the wild-type and various engineered phosphoseryl-tRNA synthetases (SepRSs). The D418N D420N T423V triple mutant of SepRS efficiently charged phosphoserine to tRNA containing the PaUA anticodon with a Km = 47.1 μM and a kcat = 0.151 sâ1, which are comparable to the values of the wild-type SepRS for its cognate substrate, tRNACys with the GCA anticodon (26.9 μM and 0.111 sâ1). The triple mutant SepRS and the tRNA with the PaUA anticodon represent a specific pair for the site-specific incorporation of phosphoserine into proteins in response to the UADs codon within mRNA.
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Authors
Ryuya Fukunaga, Yoko Harada, Ichiro Hirao, Shigeyuki Yokoyama,