Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1936340 | Biochemical and Biophysical Research Communications | 2007 | 7 Pages |
Abstract
Nectins are Ca2+ independent cell adhesion molecules localizing at the cadherin based adherens junctions. In this study, we have used atomic force microscopy to study interaction of a chimera of extra cellular fragment of nectin-1 and Fc of human IgG (nef-1) with wild type L-fibroblasts that express endogenous nectin-1 to elucidate the biophysical characteristics of homophilic nectin-1 trans-interactions at the level of single molecule. Bond strength distribution revealed three distinct bound states (or configurations) of trans-interactions between paired nectins, where each bound state has a unique unstressed off-rate and reactive compliance. Kinetic analysis of force-dependent off-rate of the bound state involving trans-interacting V-V domains between paired nectin-1 (unstressed off-rate â¼1.465 ± 0.779 sâ1, reactive compliance â¼0.143 ± 0.072 nm) was found to be closest to E-cadherin, indicating that V-V domain trans-interactions are probably necessary to initiate and promote adhesions of E-cadherin at adherens junctions (AJs).
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Authors
Sri Ram Krishna Vedula, Tong Seng Lim, Shi Hui, P. Jaya Kausalya, E. Birgitte Lane, Gunaretnam Rajagopal, Walter Hunziker, Chwee Teck Lim,