| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1936667 | Biochemical and Biophysical Research Communications | 2007 | 5 Pages |
Abstract
Using RNA interference (RNAi) to suppress gene expression, we attempted to identify tyrosine kinases involved in the extension of neurites from SH-SY5Y cells. A comprehensive analysis of gene “knock-down” profiles with small interfering RNAs (siRNAs) revealed candidate proteins that might control neurite extension. Phenotype-based screening of differentiating SH-SY5Y cells following retinoic acid (RA) stimulation indicated that twinfilin-2 is a protein that is involved in neurite outgrowth, as confirmed by morphological analysis of twinfilin-2-overexpressing cells.
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Authors
Shigeru Yamada, Eiichiro Uchimura, Takanori Ueda, Takashi Nomura, Satoshi Fujita, Kayo Matsumoto, Daniel P. Funeriu, Masato Miyake, Jun Miyake,