| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1936744 | Biochemical and Biophysical Research Communications | 2007 | 6 Pages |
Abstract
Of the three intracellular Ca2+ signalling molecules (InsP3, cADPR and NAADP) sea urchin egg homogenate has been used in the identification and characterisation of two, cADPR and NAADP. Homogenate is prepared in a Na+/Cl− substitute of N-Methyl glucamine (NMG)/gluconate. To determine how media composition affects Ca2+ release we replaced NMG with various sugars or glycine and found a dramatic improvement in InsP3 mediated Ca2+ release. Conversely the response to cADPR was diminished, whilst NAADP was unaffected. Therefore modifying media composition may be an important consideration in using homogenate to study Ca2+ release for future studies.
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Authors
Keith T. Jones, Karl Swann,