| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1936861 | Biochemical and Biophysical Research Communications | 2008 | 7 Pages |
Abstract
GPR40 is G protein-coupled receptor whose endogenous ligands have recently been identified as free fatty acids (FFAs), and it has been implicated to play an important role in FFA-mediated enhancement of glucose-stimulated insulin release. We have developed a monoclonal antibody against the extracellular domain of GPR40. Specificity of the antibody was demonstrated by immunoprecipitation and cell surface staining using GPR40-transfected cells. GPR40 immunoreactivity was highly abundant in mouse pancreatic β-cells and splenocytes, THP-1 cells, and human peripheral blood mononuclear cells. The anti-GPR40 monoclonal antibody should prove valuable for further studying the function of this nutrient sensing receptor.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Akira Hirasawa, Chisato Itsubo, Keiko Sadakane, Takafumi Hara, Shingo Shinagawa, Hanako Koga, Hiroshi Nose, Taka-aki Koshimizu, Gozoh Tsujimoto,