Article ID Journal Published Year Pages File Type
1937024 Biochemical and Biophysical Research Communications 2007 7 Pages PDF
Abstract

Terminally misfolded or unassembled proteins are degraded by the cytoplasmic ubiquitin-proteasome pathway in a process known as ERAD (endoplasmic reticulum-associated protein degradation). Overexpression of ER α1,2-mannosidase I and EDEMs target misfolded glycoproteins for ERAD, most likely due to trimming of N-glycans. Here we demonstrate that overexpression of Golgi α1,2-mannosidase IA, IB, and IC also accelerates ERAD of terminally misfolded human α1-antitrypsin variant null (Hong Kong) (NHK), and mannose trimming from the N-glycans on NHK in 293 cells. Although transfected NHK is primarily localized in the ER, some NHK also co-localizes with Golgi markers, suggesting that mannose trimming by Golgi α1,2-mannosidases can also contribute to NHK degradation.

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