Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1937453 | Biochemical and Biophysical Research Communications | 2007 | 5 Pages |
Abstract
Streptococcus pneumoniae carries a single Ser/Thr protein kinase gene stkP in its genome. Biochemical studies performed with recombinant StkP have revealed that this protein is a functional membrane-linked eukaryotic-type Ser/Thr protein kinase. Here, we demonstrate that the deletion of its extracellular domain negatively affects the stability of a core kinase domain. In contrast, the membrane anchored kinase domain and the full-length form of StkP were stable and capable of autophosphorylation. Furthermore, evidence is presented that StkP forms dimers through its transmembrane and extracellular domains.
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Authors
Petra Pallová, Kamil Hercík, Lenka Sasková, Linda Nováková, Pavel Branny,