Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1937454 | Biochemical and Biophysical Research Communications | 2007 | 7 Pages |
Abstract
The C-terminus of cryptochrome (CRY) regulates light responses in Drosophila. These include the light-dependent binding of Drosophila dCRY to the clock proteins PERIOD and TIMELESS in a yeast two-hybrid system, which we proved to be a convenient and reliable readout of the behavior of dCRY in vivo. In this study, we present a combination of in silico analysis and experimental validation in yeast, to identify novel functional motifs in the C-terminal region of dCRY. Our results suggest that linear motifs are present in this small region, which is a likely hotspot for molecular interactions.
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Authors
Matthew J. Hemsley, Gabriella M. Mazzotta, Moyra Mason, Stephane Dissel, Stefano Toppo, Mario A. Pagano, Federica Sandrelli, Flavio Meggio, Ezio Rosato, Rodolfo Costa, Silvio C.E. Tosatto,