Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1937759 | Biochemical and Biophysical Research Communications | 2007 | 6 Pages |
Abstract
The severe acute respiratory syndrome-coronavirus nucleocapsid (N) protein is involved in virus replication and modulation of cell processes. In this latter respect control may in part be achieved through the sub-cellular localisation of the protein. N protein predominately localises in the cytoplasm (the site of virus replication and assembly) but also in the nucleus/nucleolus. Using a combination of live-cell and confocal microscopy coupled to mutagenesis we identified a cryptic nucleolar localisation signal in the central part of the N protein. In addition, based on structural comparison to the avian coronavirus N protein, a nuclear export signal was identified in the C-terminal region of the protein.
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Authors
Jae-Hwan You, Mark L. Reed, Julian A. Hiscox,