| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1937871 | Biochemical and Biophysical Research Communications | 2007 | 4 Pages |
Abstract
Colorectal cancer (CRC) is one of the leading causes of cancer death in humans. In order to identify novel cancer-promoting genes in CRC, we here constructed a retroviral cDNA expression library from a CRC cell line RKO, and used it for a focus formation assay with mouse 3T3 fibroblasts, leading to the identification of 42 independent cDNAs. One of such cDNAs turned out to encode purinergic receptor P2Y, G-protein coupled, 2 (P2RY2). The oncogenic potential of P2RY2 was confirmed in vitro with the focus formation assay as well as soft agar-growth assay, and also in vivo with a tumorigenicity assay in nude mice. While our P2RY2 cDNA encodes a protein with two amino-acid substitutions compared to the reported one, we have confirmed that the wild-type P2RY2 has a strong transforming potential as well. These results indicate an unexpected role of P2RY2 in the carcinogenesis of human cancers.
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Authors
Hisashi Hatanaka, Shuji Takada, Young Lim Choi, Shin-ichiro Fujiwara, Manabu Soda, Munehiro Enomoto, Kentaro Kurashina, Hideki Watanabe, Yoshihiro Yamashita, Kentaro Sugano, Hiroyuki Mano,