Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1938626 | Biochemical and Biophysical Research Communications | 2007 | 5 Pages |
Abstract
In this report, we investigated the lateral diffusion of transforming growth factor β (TGF-β) type I receptor (TβRI) in living cells by imaging and tracking individual green fluorescent protein tagged TβRI on the cell membrane. We found that when co-expressed with TGF-β type II receptor (TβRII), the mobility of TβRI decreased significantly after TGF-β1 stimulation. However, in the cells that had been depleted of cholesterol with Nystatin or methyl-β-cyclodextrin, the diffusion rate of TβRI was not changed by TGF-β1 treatment. Our observations suggest that membrane lipid-rafts provide an environment that facilitates the association of TβRI and TβRII for cell signaling.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Xinyong Ma, Qiang Wang, Yaxin Jiang, Zeyu Xiao, Xiaohong Fang, Ye-Guang Chen,