Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1938930 | Biochemical and Biophysical Research Communications | 2006 | 7 Pages |
Abstract
Synthetic oligodeoxynucleotides containing CpG motifs (CpG ODN) have been reported to induce antimycobacterial activity both in vitro and in vivo. The present study analyzes the signals leading to CpG ODN-induced antimicrobial activity in monocytes. In this context, CpG, but not GpC, ODN induced cytosolic Ca2+ influx of extracellular origin which, in turn, activated host phospholipase D (PLD). The production of CpG-induced PLD-dependent phosphatidic acid induced the maturation of phagolysosomes and intracellular mycobacterial growth inhibition. These results show the presence of an antimicrobial pathway in monocytes, mediated by Ca2+-dependent PLD which can be useful for the exploitation of novel anti-tuberculosis immunotherapy approaches.
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Authors
Emanuela Greco, Marco De Spirito, Massimiliano Papi, Marco Fossati, Giovanni Auricchio, Maurizio Fraziano,