Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1938991 | Biochemical and Biophysical Research Communications | 2006 | 6 Pages |
Abstract
To elucidate the regulation of IL-27p28 gene, we analyzed the promoter region of the gene in DC2.4 cells with or without lipopolysaccharide (LPS)-treatment. The results indicate that a region (−648 to −364) of p28 promoter was responsible for LPS-induction. EMSA with DNA probes within the region reveals that binding of GATA motif bound proteins was decreased by LPS-treatment. We identified one of the proteins as non-POU domain-containing octamer binding protein (NonO). Taken together, LPS-induced activation of IL-27p28 gene can be accounted for by the displacement of bound NonO protein from the IL-27p28 promoter.
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Authors
Maki Kamakura, Keiko Morisawa, Hideyuki Komi, Ayako Tomatani, Fumiji Saito, Yuko Konishi, Ya Jin, Takashi Manabe, Masayuki Kuroda, Shosuke Imai, Hiroyuki Mizuguchi, Taketoshi Taniguchi,