Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1939286 | Biochemical and Biophysical Research Communications | 2007 | 8 Pages |
Abstract
The sequence of a novel cGMP-regulated, tetrameric, K+ selective channel (Sp-tetraKCNG) was discovered in the sea urchin Strongylocentrotus purpuratus. The Sp-tetraKCNG is a single polypeptide made of four KCNG domains similar to voltage-dependent Na+ and Ca2+ channels. Each KCNG domain has six transmembrane segments (S1–S6), the ion pore having the K+ selectivity signature GYGD and a cyclic nucleotide-binding domain (CNBD). This novel channel is evolutionary located between K+-selective and voltage-dependent EAG channels and voltage-independent cationic CNG channels. Bilayer reconstitutions demonstrate such a cGMP-regulated K+ selective channel in sea urchin spermatozoa.
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Authors
Blanca Estela Galindo, José Luis de la Vega-Beltrán, Pedro Labarca, Victor D. Vacquier, Alberto Darszon,