| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1939287 | Biochemical and Biophysical Research Communications | 2007 | 5 Pages |
Arginine vasopressin (AVP) induces immediate prostaglandin E2 (PGE2) production in rat 3Y1 fibroblasts. Judging from effects of several inhibitors, cytosolic phospholipase A2α (cPLA2α) and cyclooxygenase-1 (COX-1) were mainly involved in this reaction. The antagonist of vasopressin receptor V1a, and not that of V2, inhibited the AVP-induced PGE2 synthesis, indicating that AVP activates cPLA2α through V1a receptor. Treatment of 3Y1 cells with AVP resulted in transient activation of p44/42 mitogen-activated protein kinase (MAPK) and cPLA2α, and phosphatidylinositol 3-kinase (PI3K) inhibitor blocked not only AVP-induced PGE2 synthesis but also MAPK activation, suggesting that PI3K is involved in the AVP-induced MAPK and cPLA2α activation, which initiates the production of PGE2. These results suggest that PGE2 generated by the stimulation of AVP probably modulates the physiological effects of AVP.
