Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1939454 | Biochemical and Biophysical Research Communications | 2006 | 10 Pages |
Abstract
Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or β-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues in vivo.
Keywords
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Biochemistry
Authors
Ya-Hui Wang, Chung-Pin Chen, Ming-Huan Chan, Microsugar Chang, Yu-Wun Hou, Hwei-Hsien Chen, Hui-Ru Hsu, Kevin Liu, Han-Jung Lee,