Article ID Journal Published Year Pages File Type
1939523 Biochemical and Biophysical Research Communications 2007 7 Pages PDF
Abstract

A new conformational neutralizable epitope is created on heptocyte growth factor (HGF), when it interacts with its receptor, cMet. By immunizing rabbits with HGF–cMet complex, we successfully generated a monoclonal antibody (SFN68) that inhibits HGF–cMet interaction, and blocks the biological function mediated by HGF. To define the epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF–cMet interaction. No humoral response was induced to this epitope-mimicking peptide when immunization was done with HGF alone.

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