| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1940086 | Biochemical and Biophysical Research Communications | 2006 | 4 Pages |
Abstract
F0F1-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody–antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F0F1-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate that the biosensor loading of virus particles has remarkable signal-to-noise ratio (3.8:1) compared to its control at single molecular level, and will be convenient, quick, and even super-sensitive for detecting virus particles.
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Authors
XiaoLong Liu, Yun Zhang, JiaChang Yue, PeiDong Jiang, ZhenXi Zhang,