Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1941009 | Biochemical and Biophysical Research Communications | 2006 | 5 Pages |
Abstract
MicroRNAs are short (∼22 nucleotides), non-coding RNAs that play critical roles in gene regulation and may be used as rapid precise diagnostic indicators of early stages of cancer. The small size of these RNAs makes detection of multiple microRNA species in very small samples problematic. Here we investigate the parameters associated with multiplexing RT-PCR to obtain relative abundance profiles of multiple microRNAs in small sample sizes down to the amount of RNA found in a single cell.
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Authors
Kaiqin Lao, Nan Lan Xu, Vivian Yeung, Caifu Chen, Kenneth J. Livak, Neil A. Straus,