| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1941074 | Biochemical and Biophysical Research Communications | 2006 | 7 Pages |
Sphingosine 1-phosphate (S1P) is a ligand for S1P family receptors (S1P1–S1P5). Of these receptors, S1P1, S1P2, and S1P3 are ubiquitously expressed in adult mice, while S1P4 and S1P5 are tissue specific. However, little is known of their expression during embryonal development. We performed Northern blot analyses in mouse embryonal tissue and found that such expression is developmentally regulated. We also examined the expression of these receptors during primitive endoderm (PrE) differentiation of mouse F9 embryonal carcinoma (EC) cells, a well-known in vitro endoderm differentiation system. S1P2 mRNA was abundantly expressed in F9 EC cells, but little S1P1 and no S1P3, S1P4, or S1P5 mRNA was detectable. However, S1P1 mRNA expression was induced during EC-to-PrE differentiation. Studies using small interference RNA of S1P1 indicated that increased S1P1 expression is required for PrE differentiation. Thus, S1P1 may play an important function in PrE differentiation that is not substituted for by S1P2.
