Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1941775 | Biochemistry and Biophysics Reports | 2015 | 8 Pages |
Abstract
The ubiquitin ligases, SCF complexes, consist of Cul1, Skp1, Rbx1 and the substrate recognition components F-box proteins. Previous studies have reported that one of these F-box proteins, Fbl12, which is produced by Fbxl12 gene, regulates both cell cycle and differentiation. In this paper, we show that the intronic region of Fbxl12 gene acts as an alternative promoter and induces expression of a short form of Fbl12 that lacks F-box domain (Fbl12ÎF). We also found that UV irradiation increases Fbl12ÎF mRNA in cells. Finally, Fbl12ÎF may promote the subcellular localization of Fbl12 from nucleus to cytoplasm through their binding. Our data provide the possibility that Fbl12ÎF induced by alternative promoter controls the SCFFbl12 activity in response to UV stimulation.
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Authors
Fuminori Tsuruta, Jaehyun Kim, Tomomi Fukuda, Yu Kigoshi, Tomoki Chiba,