Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1945498 | Biochimica et Biophysica Acta (BBA) - Biomembranes | 2008 | 9 Pages |
Abstract
Sarcoplasmic reticulum (SR) vesicles were adsorbed on an octadecanethiol/phosphatidylcholine mixed bilayer anchored to a gold electrode, and the Ca-ATPase contained in the vesicles was activated by ATP concentration jumps in the presence of calcium ions. The resulting capacitive current transients are compared with those calculated on the basis of the enzymatic cycle of the calcium pump. This comparison provides information on the kinetics of the E2-E1 conformational change and on its pH dependence. The alteration in the current transients following ATP concentration jumps in the presence of curcumin is examined. In particular, curcumin decreases the rate of slippage of the Ca-ATPase, and at concentrations above 10 μM reduces calcium transport by this pump.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Gianluca Bartolommei, Francesco Tadini-Buoninsegni, Maria Rosa Moncelli, Rolando Guidelli,