| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1946509 | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms | 2013 | 9 Pages |
tRNA synthesis by yeast RNA polymerase III (Pol III) is down-regulated under growth-limiting conditions. This control is mediated by Maf1, a global negative regulator of Pol III transcription. Conserved from yeast to man, Maf1 was originally discovered in Saccharomyces cerevisiae by a genetic approach. Details regarding the molecular basis of Pol III repression by Maf1 are now emerging from the recently reported structural and biochemical data on Pol III and Maf1. The phosphorylation status of Maf1 determines its nuclear localization and interaction with the Pol III complex and several Maf1 kinases have been identified to be involved in Pol III control. Moreover, Maf1 indirectly affects tRNA maturation and decay. Here I discuss the current understanding of the mechanisms that oversee the Maf1-mediated regulation of Pol III activity and the role of Maf1 in the control of tRNA biosynthesis in yeast. This article is part of a Special Issue entitled: Transcription by Odd Pols.
► Phenotypes of yeast maf1 mutants. ► Family of eukaryotic Maf1 proteins. ► Regulation of the activity of yeast Maf1. ► Model of Pol III regulation by Maf1 and CK2 kinase. ► Indirect effect of Maf1 on tRNA processing and decay.
