| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1946587 | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms | 2012 | 8 Pages |
At the core of chromosome segregation is the centromere, which nucleates the assembly of a macromolecular kinetochore (centromere DNA and associated proteins) complex responsible for mediating spindle attachment. Recent advances in centromere research have led to identification of many kinetochore components, such as the centromeric-specific histone H3 variant, CenH3, and its interacting partner, Scm3. Both are essential for chromosome segregation and are evolutionarily conserved from yeast to humans. CenH3 is proposed to be the epigenetic mark that specifies centromeric identity. Molecular mechanisms that regulate the assembly of kinetochores at specific chromosomal sites to mediate chromosome segregation are not fully understood. In this review, we summarize the current literature and discuss results from our laboratory, which show that restricting the localization of budding yeast CenH3, Cse4, to centromeres and balanced stoichiometry between Scm3 and Cse4, contribute to faithful chromosome transmission. We highlight our findings that, similar to other eukaryotic centromeres, budding yeast centromeric histone H4 is hypoacetylated, and we discuss how altered histone acetylation affects chromosome segregation. This article is part of a Special Issue entitled: Chromatin in time and space.
► Hypoacetylation of centromeric H4 contributes to chromosome transmission fidelity. ► Altered histone H4 HAT and HDAC activities lead to chromosome missegregation. ► Mislocalization of Cse4 to non-centromeric regions leads to chromosome loss. ► N-terminal domain of Cse4 contributes to its exclusion from non-centromeric regions. ► Misregulation of Scm3/HJURP causes defects in chromosome segregation.
