| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1946721 | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms | 2011 | 7 Pages |
Metallothionein (MT) is a small, cysteine-rich protein active in zinc homeostasis, cadmium detoxification, and protection against reactive oxygen species. Mouse MT-I gene transcription is regulated by metal response element-binding transcription factor-1 (MTF-1), which is recruited to the promoter by zinc. We examined alterations in the chromatin structure of the MT-I promoter associated with enhanced transcriptional activation. MTF-1 proved essential for zinc-induced epigenetic changes in the MT-I promoter. Chromatin immunoprecipitation assays demonstrated that zinc treatment rapidly decreased Lys4-trimethylated and Lys9-acetylated histone H3 in the promoter and decreased total histone H3 but not histone H3.3. Micrococcal nuclease sensitivity of the MT-I promoter was increased by zinc. Thus, the chromatin structure in the promoter may be locally disrupted by zinc-induced nucleosome removal. Without MTF-1 these changes were not observed, and an MTF-1 deletion mutant recruited to the MT-I promoter by zinc that did not recruit the coactivator p300 or activate MT-I transcription did not affect histone H3 in the MT-I promoter in response to zinc. Interleukin-6, which induces MT-I transcription independently of MTF-1, did not reduce histone H3 levels in the promoter. Rapid disruption of nucleosome structure at the MT-I promoter is mediated by zinc-responsive recruitment of an active MTF-1-coactivator complex.
Research Highlights► Zn activates MTF-1 and induces metallothionein (MT) gene transcription. ► Zn disrupts chromatin structure in MT-I promoter by nucleosome removal. ► Interleukin-6 activates MT transcription with MTF-1-independent manner. ► Interleukin-6 does not disrupt the chromatin structure. ► The disruption may be mediated by MTF-1–coactivator complex.
