| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1946814 | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms | 2010 | 6 Pages |
Eukaryotic gene expression is a multilayer process covering transcription to post-translational protein modifications. As the nascent pre-mRNA emerges from the RNA polymerase II (RNAPII), it is packed in a messenger ribonucleoparticle (mRNP) whose optimal configuration is critical for the normal pre-mRNA processing and mRNA export, mRNA integrity as well as for transcription elongation efficiency. The interplay between transcription and mRNP formation feeds forward and backward and involves a number of conserved factors, from THO to THSC/TREX-2, which in addition have a unique impact on transcription-dependent genome instability. Here we review our actual knowledge of the role that these factors play at the interface between transcription and mRNA export in the model organism Saccharomyces cerevisiae.
Research Highlights► THO and THSC play different roles at transcription/mRNA export interface ► Aberrant mRNPs accumulate in THO mutants forming R-loops ► R-loops cause genome instability ► Defects in mRNP biogenesis hinder RNAPII transcription
