Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1947223 | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression | 2006 | 8 Pages |
Abstract
Cytoglobin (CYGB) is a member of the vertebrate globin family together with hemoglobin, myoglobin and neuroglobin. Although the physiological function of CYGB is still unclear, spectroscopic studies show that CYGB contains a hexacoordinated heme pocket similar to other pentacoordinated globin proteins. CYGB shares a common phylogenetic ancestry with vertebrate myoglobin from which it diverged by duplication before the appearance of jawed vertebrates. The objective of this study is to identify the regulatory and promoter region of the human cytoglobin gene. 5â² unidirectional deletion constructs demonstrated that the proximal promoter elements of human CYGB gene are located between â1113 to â10 relative to the translation start site. Site-directed mutagenesis showed that mutation of a c-Ets-1 motif at â1008 and Sp1 motifs at â400, â230 and â210 remarkably decreased the promoter activity. Gel shift assays confirmed the binding of DNA-nuclear proteins to these motifs. All these results indicate that CYGB gene expression can be up-regulated by c-Ets-1 and Sp1 motifs.
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Authors
XiuMei Guo, Sjaak Philipsen, Kian-Cheng Tan-Un,