Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1947934 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2010 | 9 Pages |
Abstract
Finally, we show, using thermal denaturation and enzymatic activity measurements, that whatever the pH value, the propeptide does not stabilize the structure of the catalytic domain but very interestingly, prevents its autolysis.
Keywords
PBSPropeptide8-anilino-1-naphthalenesulfonic acidBMGYdithiotreitolE-64BOCPROPAmCPDCDTT7-amino-4-methylcoumarinThermal denaturationDer p 1AllergenEDTASDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisStabilityFluorescence quenchingZymogenANSCysteine proteasePhosphate buffered salineMechanism of activationwild-type
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Authors
Andy Chevigné, Marie-Eve Dumez, Mireille Dumoulin, André Matagne, Alain Jacquet, Moreno Galleni,