| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1949342 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2012 | 9 Pages |
The carnitine/acylcarnitine translocase (CACT), an integral protein of the mitochondrial inner membrane, belongs to the carnitine-dependent system of fatty acid transport into mitochondria, where beta-oxidation occurs. CACT exchanges cytosolic acylcarnitine or free carnitine for carnitine in the mitochondrial matrix. The object of this study was to investigate in rat liver the effect, if any, of diets enriched with saturated fatty acids (beef tallow, BT, the control), n − 3 polyunsaturated fatty acids (PUFA) (fish oil, FO), n − 6 PUFA (safflower oil, SO), and mono-unsaturated fatty acids (MUFA) (olive oil, OO) on the activity and expression of CACT. Translocase exchange rates increased, in parallel with CACT mRNA abundance, upon FO-feeding, whereas OO-dietary treatment induced a decrease in both CACT activity and expression. No changes were observed upon SO-feeding. Nuclear run-on assay revealed that FO-treatment increased the transcriptional rate of CACT mRNA. On the other hand, only in the nuclei of hepatocytes from OO-fed rats splicing of the last intron of CACT pre-mRNA and the rate of formation of the 3′-end were affected. Overall, these findings suggest that compared to the BT‐enriched diet, the SO-enriched diet did not influence CACT activity and expression, whereas FO- and OO-feeding alters CACT activity in an opposite fashion, i.e. modulating its expression at transcriptional and post-transcriptional levels, respectively.
► Wistar rats were fed on different dietary fat types using beef tallow as control. ► Fish- and olive oil-feeding oppositely alter hepatic CACT activity and expression. ► CACT activity and expression are unaffected by safflower oil-feeding. ► Fish oil-feeding stimulates transcriptional rate of CACT gene. ► Olive oil-feeding affects splicing and 3'-end formation of CACT pre-mRNA.
