Identification of an amino acid residue in ATP-binding cassette transport G1 critical for mediating cholesterol efflux

Article ID	Journal	Published Year	Pages	File Type
1949424	Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids	2012	8 Pages	PDF

Abstract

âº Cys514 is critical for ABCG1-mediated cholesterol efflux. âº Mutating Cys514 to Ala, Thr and Ser impaired ABCG1-mediated cholesterol efflux. âº Mutation of Cys514 has no effect on ABCG1 trafficking and dimerization.

Keywords

ABCG1 DAPI ABC ApoA-I MSD RCT HEK TCA NBD DTT rHDL FBS DTBP DMEM PBS 4′,6-diamidino-2-phenylindole BSA Dulbecco's modified Eagle's medium Atherosclerosis bovine serum albumin Apolipoprotein A-I trichloroacetic acid SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis Cysteine residue ATP-binding cassette transporter Cholesterol efflux nucleotide-binding domain membrane-spanning domain transmembrane domain dithiothreitol fetal bovine serum transmembrane phosphate buffer saline reverse cholesterol transport human embryonic kidney