Endoplasmic reticulum stress-mediated inhibition of NSMase2 elevates plasma membrane cholesterol and attenuates NO production in endothelial cells

Article ID	Journal	Published Year	Pages	File Type
1949452	Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids	2012	11 Pages	PDF

Abstract

âº NSMase2 inhibition by ER stress-results in elevated cellular cholesterol. âº NO-levels in ER stressed cells were severely attenuated (~Â 70%) within 24Â h. âº NSMase2 overexpression increased eNOS phosphorylation but did not affect NO levels. âº NSMase2 knock down decreases eNOS phosphorylation (~Â 50%) and NO formation (~Â 40%).

Keywords

DAOS carboxy-H2DCFDA BAEC tunicamycin S2P GRP78 ATF6 S1P dichlorofluorescein eNOS CD36 SREBP-2 4-aminoantipyrine Neutral sphingomyelinase 2 NOA AcCh Dcf nitric oxide analyzer TCA 5-(and-6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate RNA interference Small interfering RNA RNAi ROS siRNA Endothelial dysfunction ER stress Acetylcholine neutral sphingomyelinase trichloroacetic acid Over-expression Immunoprecipitation cluster of differentiation 36 Knock-down RONS Bovine aortic endothelial cells endothelial nitric oxide synthase endoplasmic reticulum palmitate Plasma membrane activating transcription factor 6 Nitric oxide nitric oxide synthase glucose-regulated protein 78 Reactive oxygen/nitrogen species Reactive oxygen species