High throughput quantitative molecular lipidomics

Applications in biomedical research increasingly demand detailed lipid molecule information acquired at high throughput. Although the recent advances in lipidomics offer to delineate the lipidomes in detail, the challenge remains in performing such analyses at the requested quality and to maintain the quality also in a high throughput setting. In this review we describe a high throughput molecular lipidomic solution based on robotic assisted sample preparation and lipid extraction and multiple lipidomic platforms integrated with a sophisticated bioinformatics system. As demonstrated, the virtue of this lipidomic toolkit lies in its high throughput delivery of comprehensive quantitative lipidomic outputs at the molecular lipid level, its ease of scalability and its capability to serve in a regulatory setting. We anticipate that this toolkit will contribute to basic research, nutritional research and promote the discovery of new disease biomarkers, disease related mechanisms of actions and drug targets. This article is part of a Special Issue entitled Lipodomics and Imaging Mass Spectrometry.

► Molecular lipidomics. ► Robotic assisted sample preparation and lipid extraction. ► Shotgun and LC-MRM lipidomics. ► Lipidomics driven bioinformatics. ► High throughput quantitative molecular lipidomics.