| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1949522 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2011 | 8 Pages |
A study of the polar lipids of Clostridium novyi NT has revealed the presence of phosphatidylethanolamine (PE) and cardiolipin as major phospholipids with smaller amounts of phosphatidylglycerol (PG), lysyl-PG and alanyl-PG. Other minor phospholipids included phosphatidic acid, CDP-diacylglycerol, phosphatidylserine (PS) and phosphatidylthreonine (PT). PE, PG and amino acyl PG were present in both the diacyl and alk-1′-enyl acyl (plasmalogen) forms and cardiolipin plasmalogens were found to contain one or two alk-1′-enyl chains. In contrast, the precursor lipids phosphatidic acid, CDP-diacylglycerol and PS were present almost exclusively as diacyl phospholipids. These findings are consistent with the hypothesis that plasmalogens are formed from diacylated phospholipids at a late stage of phospholipid formation in Clostridium species. This novel pathway contrasts with the route in animals in which a saturated ether bond is formed at an early stage of plasmalogen biosynthesis and the alk-1-enyl bond is formed by an aerobic mechanism.
Research Highlights► The lipidome of Clostridium novyi is presented for the first time. ► The end-products of lipid biosynthesis are found in all acyl or plasmalogen species. ► The intermediates of phospholipid biosynthesis contain very little plasmalogen form. ► Plasmalogens in clostridia are formed from the corresponding all acyl lipids.
