| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1952999 | Biochimie | 2009 | 8 Pages |
Abstract
In this study the bioactivity of three differently glycosylated blood coagulation factor VII (FVII) variants (human plasma FVII, recombinant human FVII produced in CHO and BHK cell cultures) were analyzed and compared. Surface plasmon resonance studies of FVII interaction with soluble and full length TF together with FVII autoactivation assays revealed that BHK-derived FVII has the highest bioactivity, while human plasma and CHO-derived FVII showed very similar bioactivity. The affinity of FVII variants to TF correlates with FVII autoactivation rates – the higher the affinity, the faster the autoactivation rate.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Ieva Sutkeviciute, Edita Mistiniene, Jolanta Sereikaite, Vladas A. Bumelis,