Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1953250 | Biochimie | 2006 | 5 Pages |
Abstract
Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 Ã
resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*â¦H-N4(C1496) and N4*-Hâ¦O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site.
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Authors
Jiro Kondo, Boris François, Rupert J.M. Russell, James B. Murray, Eric Westhof,