Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1953495 | Biochimie | 2006 | 7 Pages |
Abstract
Suramin, a drug widely used both as a therapeutic agent and in research, inhibits translation in eukaryotic cell-free systems from rabbit reticulocyte lysate (IC50 = 142–241 μM). Suramin affects both initiation (block of 43S pre-initiation complex formation) and elongation (impairment of poly(U) translation). The drug induces an increase in the pools of ribosomal subunits and the formation of high molecular weight ribosomal complexes, thus causing the disappearance of polysomes. Ribosomes isolated from suramin-treated translating mixtures are inactivated. [3H]Suramin binds to ribosomes and to isolated 60S and 40S ribosomal subunits (116, 106 and 3 binding sites, respectively) showing higher affinity for the small subunit (Kd = 2 μM).
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Authors
Maurizio Brigotti, Roberta R. Alfieri, Pier Giorgio Petronini, Domenica Carnicelli,