| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1954540 | Biophysical Journal | 2012 | 6 Pages |
Abstract
We studied the dynamical behavior of a mononucleosome under tension using a theoretical model that takes into account the nucleosomal geometry, DNA elasticity, nonspecific DNA-protein binding, and effective repulsion between the two DNA turns. Using a dynamical Monte-Carlo simulation algorithm, we demonstrate that this model shows a behavior that for an appropriate set of parameters is in quantitative agreement with data from micromanipulation experiments on individual nucleosomes. All of the parameters of the model follow from the data obtained from two types of pulling experiments, namely, constant force and constant loading rate ensembles.
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Authors
Laleh Mollazadeh-Beidokhti, Farshid Mohammad-Rafiee, Helmut Schiessel,