Perifosine sensitizes UVB-induced apoptosis in skin cells: New implication of skin cancer prevention?

Article ID	Journal	Published Year	Pages	File Type
1963620	Cellular Signalling	2012	9 Pages	PDF

Abstract

âº Perifosine significantly enhances UVB-induced skin cell apoptosis. âº Perifosine inhibits UVB-induced Akt/mTOR and ERK/MAPK activation. âº Perifosine facilitates UVB-induced ROS and ceramide production to activate AMPK. âº Activation of AMPK promotes JNK activation in UVB and perifosine treated skin cells. âº Perifosine increases UVB-induced p53 activation to limit DNA damage.

Keywords

DMEM mTOR AMPK NAC UVB N-acetyl-l-cysteine FBS Jnk PDMP S6K1 FACS 4E-BP1 ACC mTORC1 1-Phenyl-2-decanoylamino-3-morpholino-1-propanol Ribosomal p70 S6 kinase 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Dulbecco's modified Eagle Medium MAPK mTOR complex 1 MTT ROS acetyl-CoA carboxylase ultraviolet B Apoptosis fluorescence-activated cell sorting Skin cancer fetal bovine serum UV radiation Signal transduction mammalian target of rapamycin mitogen activated protein kinase Perifosine Reactive oxygen species