| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1963626 | Cellular Signalling | 2012 | 7 Pages |
IQGAP1, an essential scaffolding protein, forms a complex with the hyaluronan receptor CD44. In this study, we have examined the importance of IQGAP1 for hyaluronan-mediated fibroblast migration and proliferation. Hyaluronan induced formation of F-actin fibers and focal adhesions, which was dependent on IQGAP1. IQGAP1 was required for hyaluronan- but not for platelet-derived growth factor (PDGF)-BB-induced cell migration, and was required for both hyaluronan- and PDGF-BB-mediated fibroblast proliferation, but not for proliferation induced by 10% fetal bovine serum. Depletion of IQGAP1 suppressed hyaluronan-induced activation of Rac1 and enhanced the activation of RhoA. Taken together, these findings indicate important roles for IQGAP1 in hyaluronan‐stimulated migration and proliferation of fibroblasts.
► IQGAP1 interacts with hyaluronan-activated CD44 in human fibroblast cultures. ► IQGAP1 expression is required for hyaluronan-stimulated migration and proliferation. ► The hyaluronan-induced activation of Rac1 is dependent on IQGAP1. ► IQGAP1 plays an important role in hyaluronan-induced actin cytoskeleton rearrangments.
