| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1963651 | Cellular Signalling | 2011 | 7 Pages |
Abstract
⺠Various mutations were introduced in helix 8 of H3R to examine its functional roles. ⺠Deletion of helix 8 abolished H3R-G-protein coupling and Gi-mediated signaling. ⺠Replacement of hydrophobic amino acids in helix 8 made H3R less activated. ⺠F7.60A was less responsive to both agonist and inverse agonist.
Keywords
PAGEHelix 8CLIC4TMDH3RGPCRERKPBSCREDMEMHEKBSAG-protein coupled receptorDulbecco's modified Eagle's mediumbovine serum albuminpolyacrylamide gel electrophoresistransmembrane domainRAMHSignalingcAMP response elementPhosphate buffered salinewild-typehistamine H3 receptorhuman embryonic kidneyextracellular signal-regulated kinase
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Atsuo Kuramasu, Jun Sukegawa, Takeya Sato, Eiko Sakurai, Takehiko Watanabe, Teruyuki Yanagisawa, Kazuhiko Yanai,