| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1965112 | Clinica Chimica Acta | 2016 | 10 Pages |
•Novel fluoroprobe RHQ was designed to be an excellent marker to estimate endogenous HOCl content.•An excess generation of endogenous ROS and HOCl is associated with diabetic dyslipidemic conditions.•Plasma advanced oxidation protein product (AOPP) or chloramines was also higher among diabetic dyslipidemic subjects.•Diabetic dyslipidemic subjects show higher myeloperoxidase activity and endogenous H2O2 content.•PBMCs grown in high glucose containing culture media also evidence enhanced endogenous ROS and HOCl.
BackgroundOxidative stress is common in type 2 diabetes. It is characterised by increased levels of reactive oxygen species (ROS), of which hypochlorous acid (HOCl) is an important component. Type 2 diabetes is characterised not only by hyperglycemia, but also by dyslipidemia. It probably underlies both the development of diabetes and also resulting complications, like cardiovascular disease. We applied a novel fluoroprobe RHQ to estimate endogenous HOCl and myeloperoxidase (MPO) activity in diabetes and dyslipidemia.MethodsOur newly designed probe, RHQ (rhodamine–quinoline based chemodosimeter) is capable of estimating endogenous HOCl selectively out of the ROS components. Isolated leukocytes from study subjects were treated with DCFDA and monocytes and neutrophils with RHQ for estimating endogenous ROS and HOCl respectively. Plasma AOPP, an indicator of HOCl was also measured. We attempted to find out the key reasons of higher HOCl content in diabetic dyslipidemic subjects by quantitating endogenous hydrogen peroxide (H2O2) and myeloperoxidase (MPO) activity.ResultsIsolated PBMCs from diabetic dyslipidemic subjects indicated enhanced ROS and HOCl generation followed by diabetic subjects without dyslipidemia and healthy controls. We explored increased production of H2O2 and enhanced enzymatic activity of myeloperoxidase (MPO) among diabetic dyslipidemic subjects (p< 0.0001) resulting in higher HOCl content.ConclusionThe hyperglycemic and hyperlipidemic challenges together enhance the production of HOCl and the fluoroprobe RHQ may be used as a novel diagnostic marker to evaluate the extent of this toxicity.
