| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1965214 | Clinica Chimica Acta | 2015 | 7 Pages |
•Th22 cells and plasma IL-22 levels were significantly decreased in AA patients.•Th17 cells were significantly lower in AA patients with normal plasma IL-17A.•Th22 cells were positively correlated with pure Th17 cells in AA patients.•Th22 cells were positively correlated with reticulocytes in AA patients.•Th22 and Th17 related molecules expressed aberrantly in AA patients.
BackgroundAplastic anemia (AA) is an immune-mediated disorder and mainly related to active destruction of hematopoietic cells by effector T lymphocytes. T helper (Th) 22 cells characterized as a novel subset of CD4 + T cells participate in the pathogenesis of autoimmune and hematological diseases. However, the role of Th22 subset in AA remains unknown.Methods31 untreated AA patients and 30 healthy controls were included in this study. The percentages of Th22, Th17 and pure Th17 cells in peripheral blood were detected by flow cytometry. ELISA to measure interleukin (IL)-22 and IL-17A plasma levels and qRT-PCR for the mRNA levels of Th22 and Th17 related molecules were performed.ResultsThe proportions of Th22, pure Th17, Th17 cells and plasma levels of IL-22 were significantly lower in untreated AA patients than those in normal controls. A positive correlation was found between Th22 and pure Th17 cells in AA. Moreover, percentages of Th22 cells correlated positively with reticulocyte counts and percentages. In addition, STAT3/STAT5 mRNA expression ratio was elevated in AA patients.ConclusionTogether, our results showed Th22 cells correlating with clinical characteristics of AA patients, indicating a possible role of Th22 immune response in the pathogenesis and therapeutic intervention of AA.
