| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1965390 | Clinica Chimica Acta | 2014 | 8 Pages |
•We analyze serum ether glycerophospholipids' molecular profiles of CAD patients.•We find lower serum level of choline plasmalogen in males with coronary stenosis.•Choline plasmalogen show high association with atherosclerosis-related parameters.•Those with oleic acid in sn− 2 show the highest association with these parameters.•Choline plasmalogen with oleic acid in sn− 2 may serve as reliable biomarkers for CAD.
BackgroundIdentifying risk factors is crucial for preventing cardiovascular events, but there are no widely accepted predictive biomarkers. In our previous study of Japanese asymptomatic cohorts, we performed global analysis of serum ether glycerophospholipids (Egp) molecular profiles, and found that choline plasmalogens (PlsCho; 1-O-alk-1′-enyl-2-acyl-sn-glycero-3-phosphocholine), particularly those containing oleic acid (18:1) in the sn− 2 position, were strongly associated with a wide range of risk factors for metabolic syndrome/atherosclerosis.MethodsWe determined serum concentrations of Egp molecular species of coronary artery disease patients (n = 50; 31 males and 19 females) by LC/MS/MS, and plasmalogen (Pls; 1-O-alk-1′-enyl-2-acyl-sn-glycerophospholipids) contents in lipoprotein fractions by HPLC using radioactive iodine.ResultsWe found that the serum concentrations of ether choline glycerophospholipids (EgpCho), particularly PlsCho, were not only significantly lower in males with significant coronary stenosis but also associated with atherosclerosis-related parameters, and their association was stronger than either high-density lipoprotein cholesterol or adiponectin. In addition, serum PlsCho containing 18:1 or linoleic acid (18:2) in sn− 2 showed the highest correlations with a wide range of atherogenic parameters among PlsCho molecular species.ConclusionThese results verify our previous findings that serum PlsCho, particularly those containing 18:1 in sn− 2, may serve as reliable biomarkers for atherosclerosis.
