| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1965879 | Clinica Chimica Acta | 2012 | 5 Pages |
BackgroundThe diagnosis for smear-negative pulmonary tuberculosis (TB) is very difficult. Proteomic fingerprinting of sera is a potentially useful tool.MethodsThis study analyzed the results of the proteomic fingerprinting in sera obtained from active TB patients and controls using the surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) and protein-chip technology. The peaks were detected and analyzed, and a diagnostic system was developed. The protein peak was identified using high performance liquid chromatography (HPLC)–tandem matrix-assisted laser desorption/ionization-TOF-MS (MALDI-TOF-MS).ResultsAround 50 protein peaks were found significantly different between the TB patients and the controls (P < 0.01). Three protein peaks at m/z 5643, 4486 and 4360 were selected for system classification and the development of a decision model. The model distinguished the TB patients from the controls with a sensitivity of 96.9% and a specificity of 97.8%, respectively. The diagnostic accuracy was up to 97.3%. The one most discrepant protein peak at m/z 5643 seen in sera of active TB patients was identified as orosomucoid.ConclusionsA diagnostic system for active TB was developed using mass spectrometry and protein chip technology and required only small-volume serum samples. One potential protein biomarker at m/z 5643 was identified as orosomucoid.
► The human sera were analyzed by mass spectrometry. ► The proteomic fingerprinting in sera was obtained. ► Around 50 significant protein peaks were found. ► Three protein peaks were selected for the development of a decision model. ► The protein peak at m/z 5643 was identified as orosomucoid.
