Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1968276 | Clinica Chimica Acta | 2006 | 4 Pages |
BackgroundElevated levels of glutathione (GSH) have been reported to play an important role in mediating chemoresistance in tumor cells. The regulation of γ-glutamylcysteine synthetase (γ-GCS) is one of the major determinants of GSH homeostasis. The aim of our study was to investigate γ-GCS gene expression in patients affected by acute myeloid leukemia (AML).MethodsA total of 64 AML samples, including 23 acute promyelocytic leukemia (APL or M3) cases, were included in the study. γ-GCS mRNA levels were determined by real-time quantitative RT-PCR. All patients were evaluated at diagnosis, whereas post-treatment γ-GCS mRNA levels were assessed at the end of the consolidation therapy in 16 cases.ResultsOur data showed that variable degrees of γ-GCS expression were detectable in AML, likely reflecting disease heterogeneity; in particular, APL cases, compared to the other AML subsets, showed both significantly lower basal levels of γ-GCS mRNA at presentation and significantly increased mRNA levels after treatment.ConclusionsDecreased levels of γ-GCS leading to reduced GSH may at least in part explain the higher sensitivity of APL to chemotherapy.