| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1968801 | Clinical Biochemistry | 2013 | 9 Pages |
RationaleAmong patients with surgically resected stage I lung adenocarcinoma, some succumb to early recurrence, while others survive for more than 5 years. Few markers to predict prognoses in these patients have been accepted. Recent advances in proteomic methodologies offer a unique chance to identify new candidate biomarkers. The aim of this study is to find differences in protein expression in resected lung cancer tissue of stage I adenocarcinoma from patients with no recurrence for more than 5 years and from those with early recurrence.MethodsLung cancer tissues were obtained from 15 patients with pathologically confirmed stage I adenocarcinoma. The patients were divided into two groups, those with recurrence within 36 months (early recurrence group, n = 9) and those that were disease-free for over 5 years (disease free group, n = 6). Tissue proteins were separated by a two-dimensional electrophoresis long gel system (30 × 40 cm) with set ranges (3-10NL) and examined by nano-LC-ESI-MS/MS. Western blot assays were performed to validate these proteins.ResultsTwelve protein spots were up-regulated and 8 were down-regulated in the disease-free group as compared with the recurrence group. Of the 12 up-regulated proteins, haptoglubin, tau-tubulin kinase-2 (TTBK2), thymidine phosphorylase, annexin-1, PIN1, CAPG, and SEC23 were validated by Western blot. Among the 8 down-regulated proteins, serpinB6 and trangelin-2 were validated.ConclusionsA total of 9 differentially expressed proteins were successfully extracted, identified, and confirmed from stage I lung adenocarcinoma tissues. The increased or decreased expression of these proteins according to prognosis may be the basis for further studies of proteomics in developing prognostic biomarkers.
► The prognosis of lung cancer remains poor despite improvements in treatment. ► We identify proteins expressed differentially in stage I lung adenocarcinomas. ► A total of 9 differentially expressed proteins were successfully identified. ► The differentially expressed proteins may be the predictive markers.
