Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1969471 | Clinical Biochemistry | 2009 | 5 Pages |
ObjectivesTo set-up a method for a direct evaluation in human serum of paraoxonase enzymatic activities, establishing a possible correlation with Q192R genotype polymorphism.Design and methods101 different human serum samples were genotyped for paraoxonase Q192R polymorphism by PCR restriction analysis, and evaluated spectrophotometrically with regard to paraoxon and 2-coumaranone hydrolytic activities. Both activities of paraoxonase were assayed, quantified through normalization by arylesterase activity, and compared with the data concerning Q/R genetic polymorphism.ResultsThe mean normalized paraoxonase activity was found to be significantly higher in RR than in QQ human sera (3.99 ± 0.6 versus 1.32 ± 0.44; P < 0.0001); instead, the 2-coumaranone hydrolysis showed an opposite trend (0.10 ± 0.02 versus 0.23 ± 0.04, in RR and QQ sera respectively; P < 0.0001).ConclusionsThese methods were successfully applied to the whole serum, suggesting a possible use of this approach for a clinically relevant phenotypic characterization.